{"title":"Inertpak","description":"\u003cp\u003eInertpak Columns \n\u003cbr\u003eThe Inertpak family of LC columns is based on a completely new type of Silica Gel which exhibits a remarkable degree of chemical inertness and stability. The Inertpak range is particularly effective in the analysis of amines, basic pharmaceuticals and other chemical species where hydrogen bonding may occur. This makes Inertpak columns suitable for the vast range of non-basic applications currently performed on older technology stationary phases. \u003c\/p\u003e\n\n\u003cp\u003eIn the reversed phase analysis of basic\/amine compounds, severe peak tailing can occur. This is due to the presence of surface silanol groups which remain after the derivatisation procedure. End capping with trimethyl siloxane groups helps to eliminate surface silanols, but is never completely successful. The material used for the Capital HPLC Inertpak column family represents an innovative solution to the problem of residual silanol interactions.\u003c\/p\u003e\n\n\u003cp\u003eSpecification \n\u003cbr\u003eShape    \n\u003cbr\u003e    Spherical  \n\u003cbr\u003eParticle Diameter    \n\u003cbr\u003e    5 micron  \n\u003cbr\u003eCarbon Loading    \n\u003cbr\u003e    C8(10.5%),  \n\u003cbr\u003e   ODS2(18%), \n\u003cbr\u003e   ODS-80‰(17.5%) \n\u003cbr\u003ePore Diameter    \n\u003cbr\u003e    ODS2 (150‰ or 80‰), Ph,C8,C4(150‰) \n\u003cbr\u003eSurface Area    \n\u003cbr\u003e    320-350m2\/g,\n\u003cbr\u003e   450m2\/g (ODS-80‰) \n\u003cbr\u003eTypical Efficiency    \n\u003cbr\u003e    75,000 plates\/m \u003c\/p\u003e\n\n\u003cp\u003eSurface silanols are known to undergo self-ionisation leading to fixed negative charges on the surface of the material. This leads to strong electrostatic interactions with the protonated regions of ionised basic molecules and results in severe peak tailing.\u003c\/p\u003e\n\n\u003cp\u003eFigure 1   \n\u003cbr\u003e Free silanol \n\u003cbr\u003e(moderately acid) \n\u003cbr\u003eFigure 2   \n\u003cbr\u003e \n\u003cbr\u003eThe tendency for a silanol group to undergo self ionisation depends on its electronic environment and is exceptionally strong when the adjacent silicon atom is substituted by a metallic impurity. \n\u003cbr\u003eFigures 1 and 2 above illustrate the stabilising effect of metal impurities on ionised silanols. \u003c\/p\u003e\n\n\u003cp\u003eExisting silica technology produces LC packing materials which contain substantial amounts of metallic impurities, notably sodium and iron which lead to the presence of highly activated silanols which contribute to peak tailing. \u003c\/p\u003e\n\n\u003cp\u003eThe inert silica used in Inertpak columns uses a synthesis route producing a high purity material with a very low metal content. Consequently residual silanols following the end capping process are inactive and thererfore do not create the interactions which cause peak tailing. \u003c\/p\u003e\n\n\u003cp\u003eIn addition to the inertness towards basic compounds, Inertpak columns can also be operated over a wider pH range than conventional materials. The above tests illustrate the good stabilitly of Inertpak in contact with agressive mobile phases at high and low pH values. \u003c\/p\u003e\n\n\u003cp\u003eNew packing materials create more stringent requirements when it comes to packing procedures. The proprietary packing protocol, designed by our development laboratory, allows us to supply columns in all formats from capillaries to preparative scale columns, exclusive to Capital HPLC. Our packing procedure, in concert with Capital HPLC's stringent quality control assures you of reproducible cost effective analysis.\u003c\/p\u003e","products":[],"url":"https:\/\/capitalanalytical.com\/collections\/inertpak.oembed","provider":"Capital Analytical","version":"1.0","type":"link"}