C8 Universal Column

CommaSil® C8 HPLC Column

CommaSil® C8 HPLC Column is crafted from high-purity silica microspheres. The unique surface group bonding and complete capping process to minimize silica hydroxyl activity. Comparing to C18 HPLC Column, the retention capacity, classical reversed-phase retention capacity, and selectivity are slightly weaker. Thus, it is more suitable for the separation of compounds with moderate or high hydrophobicity.

Separation Mode Bonded Phase Carbon Load % Pore Size (Ā) Particle Size (μm) pH Range Surface Area (m2/g) USP Code
Reverse Phase C8 12 120 3/3.5/5  2-10 300 L7

 

Determination of Mycophenolate Mofetil Related Substances

Chromatographic column: CommaSil® C8 (250×4.6 mm, 5 μm)

Cat. #: HC8536

Detector: UV detector

Detection wavelength: 250 nm

Flow rate: 1.5 ml/min

Column temperature: 45 ℃

Injection volume: 10 μL

Mobile phase: PBS (Add 2 ml of triethylamine to 650 ml of water and mix well. Adjust the pH to 5.3 by dilute phosphoric acid) : Acetonitrile = 65:35

A,B,D,E,H,F,G are impurities and API is mycophenolate mofetil.

Particle size: 3μm

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