Amino (NH2) HPLC

CommaSil® NH2 HPLC Column

CommaSil® NH2 HPLC column employs unique amino bonding technology, which effectively reduces the hydrolysis of the amino bonded phase, thus improving the stability and life of the column. The excellent retention and selectivity under normal phase, reversed phase or ion exchange conditions are suitable for the separation of a wide range of compounds. The column is not only suitable for the separation of hydrophobic compounds in normal phase mode, but also for the separation of hydrophilic and polar compounds in HILIC mode (hydrophilicity exchange liquid chromatography). In HILIC mode, the column can be used to separate polar compounds such as carbohydrates, monosaccharides, oligosaccharides, sugar alcohols, etc.

Separation Mode Bonded Phase Carbon Load % Pore Size (Ā) Particle Size (μm) pH Range Surface Area (m2/g) USP Code
Normal Phase NH2 4 130 3/3.5  2-8 300 L8

Separation of Glucosamine Sulfate Related Impurities

Chromatographic column: CommaSil® NH2 (250.4.6 mm, 5 .m)

Cat. #: HNH536

Detector: UV 195 nm

Flow rate: 1 ml/min

Column temperature: 35

In left-to-right order: N-acetylglucosamine (impurity A), fructosazine

Mobile phase: acetonitrile : buffer solution = 70:30 (V/V) (impurity B), deoxyfructosazine (impurity C), glucosamine sulfate (Buffer solution: dissolve 3.5 g dipotassium hydrogen phosphate (main substance). and 0.25 mL ammonia in water, and dilute to 1000 mL, adjust the pH to 7.5 by phosphoric acid)

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