SKU:HC8535
In stock
CommaSil HPLC C8 Universal column, 250 x 4.6mm, 3.5um
CommaSil HPLC C8 Universal column, 250 x 4.6mm, 3.5um
Pack size: each, leadtime 5-7 days
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CommaSil® C8 Universal HPLC Column
CommaSil® C8 HPLC Column
CommaSil® C8 HPLC Column is crafted from high-purity silica microspheres. The unique surface group bonding and complete capping process to minimize silica hydroxyl activity. Comparing to C18 HPLC Column, the retention capacity, classical reversed-phase retention capacity, and selectivity are slightly weaker. Thus, it is more suitable for the separation of compounds with moderate or high hydrophobicity.
| Separation Mode | Bonded Phase | Carbon Load % | Pore Size (Ā) | Particle Size (μm) | pH Range | Surface Area (m2/g) | USP Code |
| Reverse Phase | C8 | 12 | 120 | 3/3.5/5 | 2-10 | 300 | L7 |
Determination of Mycophenolate Mofetil Related Substances
Chromatographic column: CommaSil® C8 (250×4.6 mm, 5 μm)
Cat. #: HC8536
Detector: UV detector
Detection wavelength: 250 nm
Flow rate: 1.5 ml/min
Column temperature: 45 ℃
Injection volume: 10 μL
Mobile phase: PBS (Add 2 ml of triethylamine to 650 ml of water and mix well. Adjust the pH to 5.3 by dilute phosphoric acid) : Acetonitrile = 65:35
A,B,D,E,H,F,G are impurities and API is mycophenolate mofetil.
