Collection: Gel Permeation and TSK columns

TSKgel® columns are available for gel filtration, gel permeation, ion exchange, reversed phase, hydrophobic interaction, hydrophilic interaction, and affinity chromatography.

TSKgel and TOYOPEARL® are widely popular columns and packing materials for the HPLC separation of proteins, peptides, and other biomolecules. TSKgel columns are packed with either silica or methacrylate particles with pore sizes to accommodate a wide range of analyte molecular weights. Both particles feature surface chemistry designed to minimize sample adsorption. All popular biomolecule separation modes are covered by TSKgel columns, including size exclusion (gel filtration and gel permeation), ion exchange, affinity, reversed-phase, HIC (hydrophobic interaction), and HILIC (hydrophilic interaction) chromatography.

Range of products available:

TSKgel SW Series Gel Filtration Columns (Silica-Based)	TSKgel PEEK Columns	Anion-Exchange Columns	TSKgel PW Series Gel Filtration Columns (Polymer-Based)	Normal Phase / Hydrophilic Interaction (HILIC) Columns	Hydrophobic Interaction Columns	Cation-Exchange Columns	TSKgel HHR Series Gel Permeation Columns	Reversed-Phase Columns	TSKgel HXL Series Gel Permeation Columns	Affinity Columns

TSKgel SW Series Gel Filtration Columns (Silica-Based)

Column	Particle Size (µm)	Pore Size (Å)	Sample MW (globular proteins)

TSKgel Affinity Columns - Ordering Information

High efficiency, resin-based TSKgel affinity columns (10 µm particles) separate or purify many enzymes and other proteins. The table below describes the characteristics of the columns offered.

Column	Immobilized Ligand	Adsorption Capacity (per mL Gel)	Typical Uses

TSKgel Hydrophobic Interaction Columns - Ordering Information

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins.

Column	Proteins, mixture	Mobile Phase	Flow Rate	Det.