Commasil HPLC column NH2, Amino 150 x 4.6mm, 3.5um

CommaSil® NH2 HPLC columns employ unique amino bonding technology, which effectively reduces the hydrolysis of the amino bonded phase, thus improving the stability and life of the column. The excellent retention and selectivity under normal phase, reversed phase or ion exchange conditions are suitable for the separation of a wide range of compounds.

 The column is not only suitable for the separation of hydrophobic compounds in normal phase mode, but also for the separation of hydrophilic and polar compounds in HILIC
mode (hydrophilicity exchange liquid chromatography). In HILIC mode, the column can be used to separate polar compounds such as carbohydrates, monosaccharides, oligosaccharides, sugar alcohols, etc.

Specifications

Carbon loading 4%

Pore Size (Å)  130

Particle size 3.5um

pH range 2-8

Surface Area 
(m2/g)  300

USP Code L8 

Separation of Glucosamine Sulfate Related Impurities
Chromatographic column: CommaSil® NH2 (250×4.6 mm, 5 μm)
Cat. #: HNH536
Detector: UV 195 nm
Flow rate: 1 ml/min
Column temperature: 35 ℃
Mobile phase: acetonitrile : buffer solution = 70:30 (V/V)
(Buffer solution: dissolve 3.5 g dipotassium hydrogen phosphate and 0.25 mL ammonia in water, and dilute to 1000 mL, adjust the pH to 7.5 by phosphoric acid)


In left-to-right order: N-acetylglucosamine (impurity A), fructosazine (impurity B), deoxyfructosazine (impurity C), glucosamine sulfate (main substance).